题目：Novel Molecular and Mechanical Properties from Transgenic silkworm
报告人：Masao NAKAGAKI（中垣 雅雄） 教授
Department of Applied Biology
Faculty of Textile Science and Technology
时间：
地点：苏州大学独墅湖校区炳麟图书馆7楼712会议室
报告主要内容：
1. Novel Molecular and Mechanical Properties of Egg Case Silk from Wasp Spider
2. Molecular Architecture of Egg Case Silk Protein in a Spider
3. Transgenic silkworm
报告人简介
Masao NAKAGAKI（中垣 雅雄）博士。先后毕业于日本信州大学和名古屋大学，留学美国加州大学伯克利分校。现为日本国立信州大学遗传学教授。一直致力于研究丝蛋白质的分子遗传与表达调控，在家蚕转蜘蛛丝基因工作方面成绩突出，2004年在世界上率先获得1-2%蜘蛛丝成份的转基因家蚕丝，2006年获得高达11%蜘蛛丝成份的家蚕丝，2007年开始产业化应用。蜘蛛丝是一种具有特殊品质的材料，迄今为止人类还无法生产出像它那样具有超级强度和弹性的化合物，英美日和中国等国都致力研究高效生产并利用蜘蛛丝作为医疗、航天和航空材料。
PUBLICATIONS（SELECTED）
1. Zhang Yuansong; Hu Junhua; Miao Yungen; Zhao Aichun; Zhao Tianfu; Wu Dayang; Liang Liefeng; Miikura Ayumi; Shiomi Kunihiro; Kajiura Zenta; Nakagaki Masao. Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield. Molecular biology reports 2008; 35(3):329-35.
2. Shiomi Kunihiro; Fujiwara Yoshihiro; Yasukochi Yuji; Kajiura Zenta; Nakagaki Masao; Yaginuma Toshinobu. The Pitx homeobox gene in Bombyx mori: regulation of DH-PBAN neuropeptide hormone gene expression.Molecular and cellular neurosciences 2007; 34(2):209-18.
3. Y.-G. Miao, A.-C. Zhao, Y.-S. Zhang, K. Nakagaki, Y. Meng, T.-F. Zhao and M. Nakagaki*.Silkworm, Bombyx mori larvae expressed the spider silk protein through a novel Bac-to-Bac/BmNPV baculovirus.Journal of Applied Entomology , 2006,130(5):297-301.
4. Yue Wanfu; Miao Yungen; Li Xinghua; Wu Xiaofeng; Zhao Aichun; Nakagaki Masao. Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system. Applied microbiology and biotechnology 2006; 73(1):181-6.
5. Miao Yungen; Zhang Yuansong; Nakagaki Koichi; Zhao Tianfu; Zhao Aichun; Meng Yan; Nakagaki Masao; Park Enoch Y; Maenaka Katsumi. Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system. Applied microbiology and biotechnology 2006; 71(2):192-9.
6. Zhao Ai-Chun; Zhao Tian-Fu; Nakagaki Koichi; Zhang Yuan-Song; Sima Yang-Hu; Miao Yun-Gen; Shiomi Kunihiro; Kajiura Zenta; Nagata Yoko; Takadera Masayuki; Nakagaki Masao. Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi.
Biochemistry 2006; 45(10):3348-56.
7. Zhao Aichun; Zhao Tianfu; Sima Yanghu; Zhang Yuansong; Nakagaki Koichi; Miao Yungen; Shiomi Kunihiro; Kajiura Zenta; Nagata Yoko; Nakagaki Masao. Unique molecular architecture of egg case silk protein in a spider, Nephila clavata. Journal of biochemistry 2005; 138(5):593-604.
8. Shiomi Kunihiro; Fujiwara Yoshihiro; Atsumi Tsutomu; Kajiura Zenta; Nakagaki Masao; Tanaka Yoshiaki; Mizoguchi Akira; Yaginuma Toshinobu; Yamashita Okitsugu. Myocyte enhancer factor 2 (MEF2) is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm, Bombyx mori. The FEBS journal 2005; 272(15):3853-62.
9. Abo Yohichi; Hagiya Akiko; Naganuma Takao; Tohkairin Yukiko; Shiomi Kunihiro; Kajiura Zenta; Hachimori Akira; Uchiumi Toshio; Nakagaki Masao
Baculovirus-mediated expression and isolation of human ribosomal phosphoprotein P0 carrying a GST-tag in a functional state. Biochemical and biophysical research communications 2004; 322(3):814-9.
10. Shimizu Tomomi; Nakagaki Masao; Nishi Yoshinori; Kobayashi Yuji; Hachimori Akira; Uchiumi Toshio
Interaction among silkworm ribosomal proteins P1, P2 and P0 required for functional protein binding to the GTPase-associated domain of 28S rRNA.
Nucleic acids research 2002; 30(12):2620-7.
11. Uchiumi T; Nomura T; Shimizu T; Katakai Y; Mita K; Koike Y; Nakagaki M; Taira H; Hachimori A. A covariant change of the two highly conserved bases in the GTPase-associated center of 28 S rRNA in silkworms and other moths. The Journal of biological chemistry 2000; 275(45):35116-21.
(科研办公室)
