医学部系列学术讲座之十九

题目 ：Apoptosis and Mitochondria: Stepping stones on the Path of Life and Death

报告人：Douglas R. Green

Peter C. Doherty Endowed Chair of Immunology,

St. Jude Children’s Research Hospital，USA

地点：苏州大学独墅湖新校区炳麟图书馆825会议室

时间：2008年10月28日（周二）下午2点

报告人介绍：

Dr. Green于1981年获得耶鲁大学的博士学位，先后在Yale, Univ. Alberta, La Jolla Institute for Allergy and Immunology, University of California, San Diego和St. Jude Children’s Research Hospital担任职务。现为St. Jude Children’s Research Hospital免疫学系主任。他的研究涉及分子免疫学得多个领域，主要侧重于凋亡的分子机制。他的团队发表了三百多篇SCI论文，包括多篇nature, science及cell文章。他现为cancer research, JBC, cancer cell, Cytokines等刊物的副主编或编委。

最近两年的代表性文章：

1. Kiessling, S., and Green, D.R. Cell survival and proliferation in Drosophila S2 cells following apoptotic stress in the absence of the APAF-1 homology, ARK, or downstream caspases. Apoptosis, 11, 497-507, 2006.

2. Tu, S., McStay, G.P., Boucher, L.M., Mak, T., Beere, H.M., and Green, D.R. In situ “trapping” of activated initiator caspases reveals a role for caspase-2 in heat shock-induced apoptosis. Nature Cell Biol., 8, 72-77, 2006.

3. Saleh, M., Mathison, J.C., Wolinski, M.K., Bensinger,S.J., Fitzgerald, P., Ulevitch, R.J., Green, D.R., and Nicholson, D.W. (shared senior authorship) Enhanced bacterial clearance and sepsis resistance in caspase-12 deficient mice. Nature, 440, 1064-1068, 2006.

4. Maurer, U., Charvet, C., Wagman, A.S., Dejardin, E., and Green, D.R. Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of Mcl-1. Mol. Cell, 21, 749-760, 2006.

5. Green, D.R. At the gates of death. Cancer Cell, 9, 328-330, 2006.

6. Muñoz-Pinedo, C., Guío-Carrión, A., Goldstein, J.C., Fitzgerald, P., Newmeyer, D.D., and Green, D.R. Different mitochondrial inter-membrane space proteins are released during apoptosis in a manner that is coordinately initiated but can vary in duration. Proc. Natl. Acad. Sci. USA, 103, 11573-11578, 2006.

7. Spierings, D.C., Lemmens, E.E., Grewal, K., Schoenberger, S.P., and Green, D.R. Duration of CTL activation regulates IL-2 production required for autonomous clonal expansion. Eur J Immunol., 36, 1707-1717, 2006.

8. Green, D.R., and Chipuk, J.E. p53 and metabolish: Inside the TIGAR. Cell, 126, 30-32, 2006.

9. Griffith, T.S., Kazama, H., VanOosten, R.L., Earle, J.K., Hendon, J.M., Green, D.R., and Ferguson, T.A. Apoptotic cells induce tolerance by generating helpless CD8 T cells that produce TNF-related apoptosis-inducing ligand (TRAIL). J. Immunol., 178, 2679-2687, 2007.

10. Pop, C., Fitzgerald, P., Green, D.R., and Salvesen, G. The role of proteolysis in caspase-8 activation and stabilization. Biochemistry, 46, 4398-4407, 2007.

11. Colell, A., Ricci, J.E., Tait, S., Milasta, S., Maurer, U., Bouchier-Hayes, L., Fitzgerald, P., Guio-Carrion, A., Waterhouse, N.J., Li, C.W., Barbry, P., Newmeyer, D.D., Beere, H.M., and Green, D.R. GAPDH and autophagy protect cells from caspase-independent cell death. Cell, 129, 983-997, 2007.

12. Sun, M. G., Williams, J., Munoz-Pinedo, C., Perkins, G. A., Brown, J. M.^, Ellisman, M. H., Green, D. R., and Frey, T. G. Correlated three-dimensional light and electron microscopy reveals extensive transformation of the mitochondrial inner membrane during apoptosis. Nature Cell Biol., 9, 1057-1065, 2007.

13. Green, D.R. Life, death, BH3 profiles, and the salmon mousse. Cancer Cell, 12, 97-99, 2007.

14. Sanjuan, M.A., Dillon, C., Tait, S.W.G., Moshiach, S., Dorsey, F., Connell, S., Komatsu, M., Tanaka, K., Cleveland, J.L, Withoff, S., and Green, D.R. Toll-like receptor signaling in macrophages links the autophagy pathway to phagocytosis. Nature, 450, 1253-1257, 2007.

15. Bouchier-Hayes, L., Munoz-Pinedo, C., Connell, S., and Green, D.R. Measuring apoptosis at the single cell level. Methods, 44, 222-228, 2008.

16. Cassidy-Stone, A., Chipuk, J.E., Ingerman, E., Song, C., Yoo, C., Kurth, M.J., Shaw, J., Hinshaw, J., Green, D.R., and Nunnari, J. Inhibitors of the mitochondrial division dynamin reveal its role in Bax/Bak-dependent mitochondrial outer membrane permeabilization. Dev. Cell, 14, 193-204, 2008.

17. Sedelies, K.A, Ciccone, A., Clarke, C.J,, Oliaro, J., Sutton, V.R., Scott, F.L., Silke, J., Susanto, O., Green, D.R., Johnstone, R.W., Bird, P.I., Trapani, J.A., Waterhouse, N.J. Blocking granule-mediated death by primary human NK cells requires both protection of mitochondria and inhibition of caspase activity. Cell Death Differ. 15, 708-717, 2008.

18. Green, D.R. Fas Bim Boom! Immunity, 28, 141-143, 2008.

19. Chipuk, J.E., and Green, D.R. How does the Bcl-2 family engage mitochondrial outer membrane permeabilization? Trends Cell Biol., 18, 157-164, 2008.

20. Green, D.R., and McKinnon, P.J. A survivor hits the breaks. Mol. Cell, 29, 411-412, 2008.

21. Oberst, A., Bender, C., and Green, D.R. Living with death: The evolution of the mitochondrial pathway of apoptosis in animals. Cell Death Differ., 15, 1139-1146.

22. Sidi, S., Sanda, T., Kennedy, R.D., Hagen, A.T., Jette, C.A., Hoffmans, R., Pascual, J., Imamura, S., Kishi, S., Amatruda, J.F., Kanki, J.P., Green, D.R., D’Andrea, A.A., and Look, A.T. Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2 and Caspase-3. Cell, 133, 864-877, 2008.

23. Kazama, H., Ricci, J.E., Herndon, J.M., Hoppe, G., Green, D.R. and Ferguson, T.A. (Shared senior authorship). Immune tolerance induction by apoptotic cells requires caspase-dependent oxidation of HMGB1. Immunity, in press.

24. Sanjuan, M.A., and Green, D.R. Eating for good health. Linking autophagy and phagocytosis in host defense. Autophagy, 4-5, 607-611, 2008.

25. Ow, Y.L.P., Green, D.R., Hao, Z., and Mak, T.W. Cytochrome c: functions beyond respiration. Nature Rev. Mol. Cell Biol., 9, 532-542.