报告时间：2010年4月7日（星期三）下午3：00
报告地点：苏州大学独墅湖校区403号楼3119室
报告人：DR. JUN-FENG WANG（王俊锋 博士）
报告人简介：
王俊锋，Position: associate professor ，Department of Psychiatry, University of British Columbia（大不列颠哥伦比亚大学精神科学系）
Research Description（主要研究方向）：
My research is on the mechanism of action of mood stabilizing drugs at molecular and cellular levels, with focus on the neuroprotective effects of these drugs against oxidative stress. Previously,we found that mood stabilizing drugs prevented oxidative damage and upregulated glutathione (GSH)/glutathione s-transferase (GST) detoxification pathway. My research objective is to understand the role of GSH/GST pathway in the pharmacological treatment of mood stabilizing drugs. Research aims are: 1) to examine if GSH and GST mediate the neuroprotective effects of mood stabilizing drugs against oxidative damage; 2) to determine the role of Nrf2 transcription factor in GSH/GST pathway regulated by mood stabilizing drugs; 3) to identify lipid peroxidation product 4-HNE oxidized protein targets and 4) to determine role of GSH/GST pathway in mood stabilizing treatment using animal models for depression and mania. This study will lead to identifying new therapeutic targets in the GSH/GST pathway for investigation in clinical trials in the near future, contributing to new and more effective therapies for bipolar disorder withfewer side effects.
Research techniques employed in my work include cell culturing, measurementof lipid peroxidation and protein oxidation, TUNEL staining, real time-PCR,DNA cloning, in vitro transcription and translation, cell transfection,immunoblotting analysis, immunohistochemistry, electrophoretic mobility shift assay, locomotor activity analysis and forced swim test among others.
Selected References(近年主要发表论文):
Wang JF, Shao L, Sun X, Young LT. Increased oxidative stress in the anterior cingulate cortex of subjects with bipolar disorder and schizophrenia. Bipolar Disord. 2009 11(5):523-9.
Johnson SA, Wang JF, Sun X, McEwen BS, Chattarji S, Young LT. Lithium treatment prevents stress-induced dendritic remodeling in the rodent amygdala.Neuroscience. 2009 29;163(1):34-9.
Tseng M, Alda M, Xu L, Sun X, Wang JF, Grof P, Turecki G, Rouleau G, Young LT. BDNF protein levels are decreased in transformed lymphoblasts from lithium-responsive patients with bipolar disorder. J Psychiatry Neurosci. 2008 33(5):449-53.
Shao L, Cui J, Young LT and Wang JF (2008). The effect o fmood stabilizer lithium on expression and activity of glutathiones-transferase isoenzymes. Neuroscience, 151: 518-24.
Wang JF (2007).Defects of Mitochondrial Electron Transport Chain in Bipolar Disorder: Implications for Mood Stabilizing Treatment. Canadian Journal of Psychiatry,52:753-62. (Review)
Cui J, Shao L, Young LT and Wang JF (2007). Role ofglutathione in neuroprotective effects of mood stabilizing drugs lithium and valproate. Neuroscience, 144: 1447-53.
Sun X, Wang JF,Tseng M and Young LT (2006). Down regulation in components of mitochondrialelectron transport chain in post-mortem frontal cortex from subjects withbipolar disorder. Journal of Psychiatry and Neuroscience, 31:189-96.
(基础医学与生物科学学院)
