报告题目：Isolation, culture and manipulation of zebrafish germ line stem cells
报告 人： Prof. Paul Collodi,
报告时间：2012年9月26日 (星期三) 9:30 AM
报告地点：703楼1楼生物钟研究中心会议室
报告人简介
The general interest of the Collodi lab is to use the zebrafish as a model to study extracellular factors that regulate early vertebrate development. The zebrafish possesses several favorable characteristics including a short generation time, external fertilization and optically clear embryos that make it an excellent model for studies involving the early stages of embryogenesis. Despite these advantages, one deficiency of the zebrafish model is the lack of methods for targeted gene inactivation and the production of knockouts. The Collodi team has been working to address this problem by developing gene-targeting methods using pluripotent embryonic stem (ES) cell and primordial germ cell (PGC) lines derived from zebrafish embryos. Methods have been established for the culture of zebrafish ES cells that possess the capacity to contribute to the germ cell lineage of a host embryo. The ES cell cultures have been used to generate transgenic lines of fish and we are currently working to use the cultures to introduce targeted mutations by homologous recombination and produce zebrafish knockouts. The ES cell cultures will enhance the value of the zebrafish model for studies of gene function. Prof. Collodi was the inaugural Editor-in-Chief for Zebrafish, a only journal primarily focused on the zebrafish research.
代表性论文：
1. Wong,TT,Saito,T, Crodian, J. and Collodi, P. (2011) Zebrafish Germline Chimeras Produced by Transplantation of Ovarian Germ Cells into Sterile Host Larvae. Biology of Reproduction 84, 1190-1197.
2. Liu, W. and Collodi, P. (2010) A zebrafish cell culture assay for the identification of microRNA targets. Zebrafish 4, 343-348.
3. Liu, W. and Collodi, P. (2010) Zebrafish dead end possesses ATPase activity that is required for primordial germ cell development. FASEB J. 24, 2641-2650.
4. Fan, L. and Collodi, P. (2008) Zebrafish primordial germ cell cultures derived from vasa::rfp transgenic embryos. Stem Cells and Development 17, 585-597.
5. Sun, L., Zou, Z., Collodi, P., Xu, F., Xu, X. and Zhao, Q. (2005) Identification and characterization of a second fibronectin gene in zebrafish. Matrix Biology 24, 69-77.
6. Zhao, Q., Liu, X. and Collodi, P. (2001) Characterization and expression of a novel fibronectin in zebrafish. Experimental Cell Research, 268, 211-219.
7. Ma, C., Fan, L., Ganassin, R., Bols, N. and Collodi, P. (2001) Production of zebrafish germ line chimeras from embryo cell cultures. Proceedings of the
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